Grizzly Analytical

Agilent 6560 Ion Mobility Q-TOF LC/MS

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Agilent 6560 Ion Mobility Quadrupole Time-of-Flight (IM-QTOF) LC/MS system with Agilent 1260/1290 frontend enables high-performance ion mobility and very precise collision cross section measurements without class dependent calibration standards. The innovative ion funnel technology in this instrument dramatically increases the ion sampling into the mass spectrometer and results in higher quality MS/MS spectra at trace levels.

Ion Mobility Q-TOF Mode Specifications

Parameter

Measure

Specification

Sensitivity, MS mode, electrospray

on-column, 200 µL/min flow rate, max sensitivity mode

200 fg LC/MS injection of reserpine. Signal for reserpine (M+H)+ at 609.2807 m/z

20 % RSD*

Drift resolution

Single charged compound

Greater than 50

Collisional cross section accuracy, MS mode

Measured without external standards

< 2 %

* Signal-to-noise (S/N) ratio is not a valid measure of sensitivity, as the Agilent 6560 can separate the reserpine signal completely away from the background noise, creating an infinite S/N calculation. Reproducibility close to the limit of detection is a more appropriate measure.

Q-TOF Mode Specifications

Parameter

Measure

Specification

Sensitivity, MS mode, electrospray on-column, 400 µL/min flow rate, and max sensitivity mode

1 pg LC/MS injection of reserpine.

S/N for the reserpine (M+H)+ at 609.2807 m/z

50:1 RMS

Sensitivity, MS/MS mode, electrospray on-column, 400 µL/min flow rate, and max sensitivity mode

1 pg LC/MS injection of reserpine. S/N for most intense product ions (174, 195,397,448 m/z)

250:1 RMS

Mass resolving power

Measured at 322 m/z after automatic tuning procedure Measured at 2,722 m/z after automatic tuning procedure

> 42,000 at 2,722 m/z

Mass accuracy – MS mode, electrospray on-column, 400 µL/min

Measured at the (M+H)+ ion of reserpine (609.2807 m/z) using an internal mass reference

< 1 ppm RMS

Mass accuracy – MS/MS mode, electrospray on-column, 400 µL/min

Product ion 397 m/z for reserpine

< 2 ppm RMS

Dynamic range

Intrascan dynamic range on coeluting components

105

Mass range

100–10,000 m/z extended mass range, 50–1,700 or 100–3,200 m/z for both high resolution and extended dynamic range modes, quadrupole up to 4,000 m/z

Spectral acquisition rate, MS mode

50 to 1,700 m/z in MS mode while maintaining a resolution of 40,000 at 1,522 m/z in 4 GHZ mode

50 spectra/second

Spectral acquisition rate, MS/MS mode

50 to 1,700 m/z in MS/MS mode while maintaining a resolution

30 MS/MS spectra/second

of 40,000 at 1,522 m/z in 4 GHZ mode

All specifications are achieved in manufacturing, and instrument performance data are supplied with shipment. All specification values are achieved after autotune, and do not require manual optimization. These specifications are not standard installation specifications for the Agilent Q-TOF. The Agilent high-resolution accurate mass Q-TOF instruments are tested and installed in accordance with standard performance tests, as described in the Agilent installation manual.

 

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