Ion Mobility Q-TOF Mode Specifications
|
Parameter
|
Measure
|
Specification
|
|
Sensitivity, MS mode, electrospray
on-column, 200 µL/min flow rate, max sensitivity mode
|
200 fg LC/MS injection of reserpine. Signal for reserpine (M+H)+ at 609.2807 m/z
|
20 % RSD*
|
|
Drift resolution
|
Single charged compound
|
Greater than 50
|
|
Collisional cross section accuracy, MS mode
|
Measured without external standards
|
< 2 %
|
* Signal-to-noise (S/N) ratio is not a valid measure of sensitivity, as the Agilent 6560 can separate the reserpine signal completely away from the background noise, creating an infinite S/N calculation. Reproducibility close to the limit of detection is a more appropriate measure.
Q-TOF Mode Specifications
|
|
|
Parameter
|
Measure
|
Specification
|
|
Sensitivity, MS mode, electrospray on-column, 400 µL/min flow rate, and max sensitivity mode
|
1 pg LC/MS injection of reserpine.
S/N for the reserpine (M+H)+ at 609.2807 m/z
|
50:1 RMS
|
|
Sensitivity, MS/MS mode, electrospray on-column, 400 µL/min flow rate, and max sensitivity mode
|
1 pg LC/MS injection of reserpine. S/N for most intense product ions (174, 195,397,448 m/z)
|
250:1 RMS
|
|
Mass resolving power
|
Measured at 322 m/z after automatic tuning procedure Measured at 2,722 m/z after automatic tuning procedure
|
> 42,000 at 2,722 m/z
|
|
Mass accuracy – MS mode, electrospray on-column, 400 µL/min
|
Measured at the (M+H)+ ion of reserpine (609.2807 m/z) using an internal mass reference
|
< 1 ppm RMS
|
|
Mass accuracy – MS/MS mode, electrospray on-column, 400 µL/min
|
Product ion 397 m/z for reserpine
|
< 2 ppm RMS
|
|
Dynamic range
|
Intrascan dynamic range on coeluting components
|
105
|
|
Mass range
|
|
100–10,000 m/z extended mass range, 50–1,700 or 100–3,200 m/z for both high resolution and extended dynamic range modes, quadrupole up to 4,000 m/z
|
|
Spectral acquisition rate, MS mode
|
50 to 1,700 m/z in MS mode while maintaining a resolution of 40,000 at 1,522 m/z in 4 GHZ mode
|
50 spectra/second
|
|
Spectral acquisition rate, MS/MS mode
|
50 to 1,700 m/z in MS/MS mode while maintaining a resolution
|
30 MS/MS spectra/second
|
of 40,000 at 1,522 m/z in 4 GHZ mode
All specifications are achieved in manufacturing, and instrument performance data are supplied with shipment. All specification values are achieved after autotune, and do not require manual optimization. These specifications are not standard installation specifications for the Agilent Q-TOF. The Agilent high-resolution accurate mass Q-TOF instruments are tested and installed in accordance with standard performance tests, as described in the Agilent installation manual.
